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Performance of the multiplex-assay on clinical trial samples. a , b Agreement with previously measured gametocyte densities with single assays, using <t>Pfs25</t> ( a ) and PfMGET ( b ) transcripts. The linear regression was fitted as follows [95% confidence interval]: for FG y = 0.9944 [0.9497–1.040]x + 0.0751 [− 0.0627 to 0.2130] , y = 1.033 [0.977–1.092]x − 0.2554 [0.4619–0.04893] and for MG y = 0.8626 [0.7744–0.9507]x + 0.5919 [0.2802–0.9037] , y = 0.9217 [0.8633–0.9800]x + 0.3508 [0.1515–0.5500] . c Gametocyte prevalence as determined by single or multiplex qPCR for four treatment arms (Dicko et al. 2018), 7 days after treatment with either DP, dihydroartemisinin–piperaquine (n = 15); DP + MB, dihydroartemisinin–piperaquine + methylene blue (n = 19); SP–AQ, sulfadoxine–pyrimethamine and amodiaquine (n = 19) or SP–AQ and a single dose of primaquine (n = 19). d Sex ratios of gametocytes determined by multiplex or individual qPCR; samples as in c
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Performance of the multiplex-assay on clinical trial samples. a , b Agreement with previously measured gametocyte densities with single assays, using <t>Pfs25</t> ( a ) and PfMGET ( b ) transcripts. The linear regression was fitted as follows [95% confidence interval]: for FG y = 0.9944 [0.9497–1.040]x + 0.0751 [− 0.0627 to 0.2130] , y = 1.033 [0.977–1.092]x − 0.2554 [0.4619–0.04893] and for MG y = 0.8626 [0.7744–0.9507]x + 0.5919 [0.2802–0.9037] , y = 0.9217 [0.8633–0.9800]x + 0.3508 [0.1515–0.5500] . c Gametocyte prevalence as determined by single or multiplex qPCR for four treatment arms (Dicko et al. 2018), 7 days after treatment with either DP, dihydroartemisinin–piperaquine (n = 15); DP + MB, dihydroartemisinin–piperaquine + methylene blue (n = 19); SP–AQ, sulfadoxine–pyrimethamine and amodiaquine (n = 19) or SP–AQ and a single dose of primaquine (n = 19). d Sex ratios of gametocytes determined by multiplex or individual qPCR; samples as in c
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Promega 0.01% rq1 dnase
Performance of the multiplex-assay on clinical trial samples. a , b Agreement with previously measured gametocyte densities with single assays, using <t>Pfs25</t> ( a ) and PfMGET ( b ) transcripts. The linear regression was fitted as follows [95% confidence interval]: for FG y = 0.9944 [0.9497–1.040]x + 0.0751 [− 0.0627 to 0.2130] , y = 1.033 [0.977–1.092]x − 0.2554 [0.4619–0.04893] and for MG y = 0.8626 [0.7744–0.9507]x + 0.5919 [0.2802–0.9037] , y = 0.9217 [0.8633–0.9800]x + 0.3508 [0.1515–0.5500] . c Gametocyte prevalence as determined by single or multiplex qPCR for four treatment arms (Dicko et al. 2018), 7 days after treatment with either DP, dihydroartemisinin–piperaquine (n = 15); DP + MB, dihydroartemisinin–piperaquine + methylene blue (n = 19); SP–AQ, sulfadoxine–pyrimethamine and amodiaquine (n = 19) or SP–AQ and a single dose of primaquine (n = 19). d Sex ratios of gametocytes determined by multiplex or individual qPCR; samples as in c
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Promega rq1 probes for southern blot and rna in situ dnase
Performance of the multiplex-assay on clinical trial samples. a , b Agreement with previously measured gametocyte densities with single assays, using <t>Pfs25</t> ( a ) and PfMGET ( b ) transcripts. The linear regression was fitted as follows [95% confidence interval]: for FG y = 0.9944 [0.9497–1.040]x + 0.0751 [− 0.0627 to 0.2130] , y = 1.033 [0.977–1.092]x − 0.2554 [0.4619–0.04893] and for MG y = 0.8626 [0.7744–0.9507]x + 0.5919 [0.2802–0.9037] , y = 0.9217 [0.8633–0.9800]x + 0.3508 [0.1515–0.5500] . c Gametocyte prevalence as determined by single or multiplex qPCR for four treatment arms (Dicko et al. 2018), 7 days after treatment with either DP, dihydroartemisinin–piperaquine (n = 15); DP + MB, dihydroartemisinin–piperaquine + methylene blue (n = 19); SP–AQ, sulfadoxine–pyrimethamine and amodiaquine (n = 19) or SP–AQ and a single dose of primaquine (n = 19). d Sex ratios of gametocytes determined by multiplex or individual qPCR; samples as in c
Rq1 Probes For Southern Blot And Rna In Situ Dnase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega rna qualified-dnase treatment rq1 dnase
Performance of the multiplex-assay on clinical trial samples. a , b Agreement with previously measured gametocyte densities with single assays, using <t>Pfs25</t> ( a ) and PfMGET ( b ) transcripts. The linear regression was fitted as follows [95% confidence interval]: for FG y = 0.9944 [0.9497–1.040]x + 0.0751 [− 0.0627 to 0.2130] , y = 1.033 [0.977–1.092]x − 0.2554 [0.4619–0.04893] and for MG y = 0.8626 [0.7744–0.9507]x + 0.5919 [0.2802–0.9037] , y = 0.9217 [0.8633–0.9800]x + 0.3508 [0.1515–0.5500] . c Gametocyte prevalence as determined by single or multiplex qPCR for four treatment arms (Dicko et al. 2018), 7 days after treatment with either DP, dihydroartemisinin–piperaquine (n = 15); DP + MB, dihydroartemisinin–piperaquine + methylene blue (n = 19); SP–AQ, sulfadoxine–pyrimethamine and amodiaquine (n = 19) or SP–AQ and a single dose of primaquine (n = 19). d Sex ratios of gametocytes determined by multiplex or individual qPCR; samples as in c
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Performance of the multiplex-assay on clinical trial samples. a , b Agreement with previously measured gametocyte densities with single assays, using Pfs25 ( a ) and PfMGET ( b ) transcripts. The linear regression was fitted as follows [95% confidence interval]: for FG y = 0.9944 [0.9497–1.040]x + 0.0751 [− 0.0627 to 0.2130] , y = 1.033 [0.977–1.092]x − 0.2554 [0.4619–0.04893] and for MG y = 0.8626 [0.7744–0.9507]x + 0.5919 [0.2802–0.9037] , y = 0.9217 [0.8633–0.9800]x + 0.3508 [0.1515–0.5500] . c Gametocyte prevalence as determined by single or multiplex qPCR for four treatment arms (Dicko et al. 2018), 7 days after treatment with either DP, dihydroartemisinin–piperaquine (n = 15); DP + MB, dihydroartemisinin–piperaquine + methylene blue (n = 19); SP–AQ, sulfadoxine–pyrimethamine and amodiaquine (n = 19) or SP–AQ and a single dose of primaquine (n = 19). d Sex ratios of gametocytes determined by multiplex or individual qPCR; samples as in c

Journal: Malaria Journal

Article Title: A multiplex assay for the sensitive detection and quantification of male and female Plasmodium falciparum gametocytes

doi: 10.1186/s12936-018-2584-y

Figure Lengend Snippet: Performance of the multiplex-assay on clinical trial samples. a , b Agreement with previously measured gametocyte densities with single assays, using Pfs25 ( a ) and PfMGET ( b ) transcripts. The linear regression was fitted as follows [95% confidence interval]: for FG y = 0.9944 [0.9497–1.040]x + 0.0751 [− 0.0627 to 0.2130] , y = 1.033 [0.977–1.092]x − 0.2554 [0.4619–0.04893] and for MG y = 0.8626 [0.7744–0.9507]x + 0.5919 [0.2802–0.9037] , y = 0.9217 [0.8633–0.9800]x + 0.3508 [0.1515–0.5500] . c Gametocyte prevalence as determined by single or multiplex qPCR for four treatment arms (Dicko et al. 2018), 7 days after treatment with either DP, dihydroartemisinin–piperaquine (n = 15); DP + MB, dihydroartemisinin–piperaquine + methylene blue (n = 19); SP–AQ, sulfadoxine–pyrimethamine and amodiaquine (n = 19) or SP–AQ and a single dose of primaquine (n = 19). d Sex ratios of gametocytes determined by multiplex or individual qPCR; samples as in c

Article Snippet: Total nucleic acids were used for the intron-containing genes SBP - 1 , Pfg14 - 744 , Pfg14 - 748 , CCp4 and PfMGET while Pfs16 , Pfg27 and Pfs25 required RQ1 DNase I treatment (Promega). cDNA was prepared with the High Capacity cDNA Reverse Transcription Kit (Applied BioSystems) and 2 μL of cDNA was run in the GoTaq qPCR Master Mix (Promega).

Techniques: Multiplex Assay

Primer and probe sequences for qRT-PCR assays, with references for earlier reported methods and primers

Journal: Malaria Journal

Article Title: A multiplex assay for the sensitive detection and quantification of male and female Plasmodium falciparum gametocytes

doi: 10.1186/s12936-018-2584-y

Figure Lengend Snippet: Primer and probe sequences for qRT-PCR assays, with references for earlier reported methods and primers

Article Snippet: Total nucleic acids were used for the intron-containing genes SBP - 1 , Pfg14 - 744 , Pfg14 - 748 , CCp4 and PfMGET while Pfs16 , Pfg27 and Pfs25 required RQ1 DNase I treatment (Promega). cDNA was prepared with the High Capacity cDNA Reverse Transcription Kit (Applied BioSystems) and 2 μL of cDNA was run in the GoTaq qPCR Master Mix (Promega).

Techniques: